{"id":4459,"date":"2023-06-01T11:56:24","date_gmt":"2023-06-01T09:56:24","guid":{"rendered":"https:\/\/www.plaisant.eu\/animal-facility\/zebrafish\/"},"modified":"2023-09-21T11:52:23","modified_gmt":"2023-09-21T09:52:23","slug":"zebrafish","status":"publish","type":"page","link":"https:\/\/www.plaisant.eu\/en\/animal-facility\/zebrafish\/","title":{"rendered":"Zebrafish"},"content":{"rendered":"

<\/div><\/div><\/div>
<\/div>

The Zebrafish animal facility is authorised for breeding, use and supply services<\/span><\/span><\/h3><\/header>
<\/div>
<\/p>\n

The facility is equipped with a Stand-Alone multi-rack system.<\/em><\/strong><\/p>\n

It has a capacity of about 900 adult fish, distributed in 2 typologies of tanks on 5 different racks. The Stand-Alone is equipped with 1 filter, 1 silicone blower (which regulates filtered and treated water input \u2013 10% daily replacement) and 1 syphon (which regulates water output and potential overflow).<\/p>\n

The Stand-Alone is refilled with osmotized (treated) water via an external apparatus (reverse osmosis water system), and decontaminated\/treated within the system itself by means of a drum filter net and a UV lamp.<\/p>\n

The system is equipped with an automatic temperature monitoring, water conductivity maintenance using synthetic salts \u201cInstant Ocean\u201d, and a constant pH monitoring and maintenance through addition of sodium bicarbonate.<\/p>\n

\n<\/div>

<\/div><\/div><\/div><\/div>
<\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section>
<\/div><\/div><\/div>
<\/div>
<\/p>\n

Services offered:<\/p>\n

    \n
  • Management of the entire breeding, maintenance and supply services<\/li>\n
  • Supervision, census, annotation and management of fish (livestock)<\/li>\n
  • Breeding of WT or Tg lines and screening of eggs by fluorescence stereomicroscope<\/li>\n
  • Genotyping<\/li>\n
  • Microinjections<\/li>\n
  • Technical-scientific experimental support<\/li>\n
  • Health monitoring according to FELASA guidelines<\/li>\n
  • Veterinary control and support<\/li>\n
  • Support for compiling Annex VI, OPBA evaluation, submission to the Ministry<\/li>\n<\/ul>\n

    \n<\/div>

    <\/div><\/div><\/div><\/div>
    <\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section>
    <\/div><\/div><\/div>
    <\/div>

    ARE YOU HAVING TROUBLES GETTING YOUR MURINE MODEL RESEARCH PROJECT APPROVED? <\/span>Try a Complementary Approach!<\/span><\/span><\/h3><\/header>
    <\/div>
    <\/p>\n

    Try our pilot studies with the emerging animal model Zebrafish\u00a0(Danio rerio). <\/em>Experiments on larvae up to 5 days post fertilisation do not require authorizations*, as they are equivalent to an in vitro cell test, however zebrafish larvae allow for observations of biological processes with the complexity of a living organism!<\/p>\n

    (*European Parliament 2010\/63\/EU Directive on the protection of animals used for scientific purposes).<\/p>\n

    \n<\/div>

    <\/div><\/div><\/div><\/div>
    <\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section>
    <\/div><\/div><\/div>
    <\/div>
    <\/p>\n

    This will allow you to:<\/p>\n

      \n
    1. get preliminary results<\/strong> that will show you the point of connection between data obtained from in vitro cell cultures and the murine model;<\/li>\n
    2. reduce the number of animals used<\/strong> in later stages of the project, thanks to an upstream screening that will direct your studies;<\/li>\n
    3. substitute<\/strong>, at least partially, the murine model with a lower encephalic neurophysiological development model<\/strong> in accordance with the principle of 3R **;<\/li>\n
    4. facilitate the process<\/strong> of ministerial approval.<\/li>\n<\/ol>\n

      (**: LEGISLATIVE DECREE 4 March 2014, No. 26. Implementation of the 2010\/63\/UE Directive on the protection of animals used for scientific purposes.)<\/p>\n

      \n<\/div>

      <\/div><\/div><\/div><\/div>
      <\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section>
      <\/div><\/div><\/div>
      <\/div>
      More and more groups around the world are using the Zebrafish model!<\/div><\/header>
      <\/div>
      \"Plaisant<\/span><\/div>
      <\/div><\/div><\/div><\/div>
      <\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section>
      <\/div><\/div><\/div>
      <\/div>
      <\/span>
      Testing in larvae for up to 5 days<\/div><\/div>
      \n
        \n
      1. Fish Embryo Acute Toxicity (FET) Test \u2013 n\u00b0 236 (26 July 2013)<\/li>\n
      2. Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages \u2013 n\u00b0 212 (21 September 1998)<\/li>\n
      3. Tap-Elicited Startle Reflex<\/li>\n
      4. Conditioned Place Preference Test<\/li>\n<\/ol>\n<\/div>\n<\/div><\/div>
        <\/span>
        Toxicity tests<\/div><\/div>
        \n
          \n
        1. Diving Test<\/li>\n
        2. Tap-Elicited Startle Reflex<\/li>\n
        3. Conditioned Place Preference Test<\/li>\n
        4. Bite Test<\/li>\n
        5. Novel tank test<\/li>\n
        6. Social test<\/li>\n
        7. Light\/dark test<\/li>\n<\/ol>\n<\/div>\n<\/div><\/div>
          <\/span>
          Behavioural Tests<\/div><\/div>
          \n
            \n
          1. Fish, Early-life Stage Toxicity Test \u2013 n\u00b0 210 (26 July 2013)<\/li>\n
          2. Fish Short Term Reproduction Assay \u2013 n\u00b0 229 (02 October 2012)<\/li>\n
          3. Fish Sexual Development Test \u2013 n\u00b0 234 (28 July 2011)<\/li>\n
          4. 21-day Fish Assay \u2013 n\u00b0 230 (8 September 2009)<\/li>\n
          5. Fish, Juvenile Growth Test \u2013 n\u00b0 215 (21 January 2000)<\/li>\n
          6. Fish, Acute Toxicity Test \u2013 n\u00b0 203 (17 July 1992)<\/li>\n<\/ol>\n<\/div>\n<\/div><\/div><\/div>
            <\/div><\/div><\/div><\/div>
            <\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section>
            \"Plaisant<\/span><\/div><\/div><\/div><\/div>
            \"Plaisant<\/span><\/div><\/div><\/div><\/div>
            <\/div>
            \"Plaisant<\/span><\/div><\/div><\/div><\/div><\/div><\/div>
            \"Plaisant<\/span><\/div><\/div><\/div><\/div>
            \"Plaisant<\/span><\/div><\/div><\/div><\/div>
            <\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":4338,"parent":4457,"menu_order":6,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"_links":{"self":[{"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/pages\/4459"}],"collection":[{"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/comments?post=4459"}],"version-history":[{"count":4,"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/pages\/4459\/revisions"}],"predecessor-version":[{"id":4627,"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/pages\/4459\/revisions\/4627"}],"up":[{"embeddable":true,"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/pages\/4457"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/media\/4338"}],"wp:attachment":[{"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/media?parent=4459"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}