{"id":4463,"date":"2023-06-01T23:50:47","date_gmt":"2023-06-01T21:50:47","guid":{"rendered":"https:\/\/www.plaisant.eu\/servizi\/ivis-unit\/"},"modified":"2024-09-17T17:04:16","modified_gmt":"2024-09-17T15:04:16","slug":"ivis-unit","status":"publish","type":"page","link":"https:\/\/www.plaisant.eu\/en\/services\/ivis-unit\/","title":{"rendered":"IVIS Unit"},"content":{"rendered":"<p><section id=\"bt_bb_section69daac9946c9b\"  class=\"bt_bb_section bt_bb_color_scheme_16 bt_bb_layout_wide bt_bb_vertical_align_top\"   data-bt-override-class=\"null\"><div class=\"bt_bb_port\"><div class=\"bt_bb_cell\"><div class=\"bt_bb_cell_inner\"><div class=\"bt_bb_row bt_bb_row_push_right bt_bb_row_push_left bt_bb_content_wide bt_bb_row_width_boxed_1200 bt_bb_row_width_boxed  bt_bb_animation_fade_in move_right animate\"  data-bt-override-class=\"{}\"><div class=\"bt_bb_row_holder\" ><div data-bb-version=\"4.5.4\"  class=\"bt_bb_column col-xxl-3 col-xl-3 bt_bb_vertical_align_top bt_bb_align_left btLazyLoadBackground bt_bb_column_background_image bt_bb_padding_normal bt_bb_details_right-inner bt_bb_details-color_white\" style=\"position: relative;background-image:url(&#039;https:\/\/www.plaisant.eu\/wp-content\/plugins\/bold-page-builder\/img\/blank.gif&#039;);; --column-width:3;\" data-background_image_src='https:\/\/www.plaisant.eu\/wp-content\/uploads\/2023\/06\/PLAISANT-ZEBRAFISH-11.jpg' data-width=\"3\" data-bt-override-class=\"{&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_left&quot;,&quot;def&quot;:&quot;left&quot;},&quot;bt_bb_padding_&quot;:{&quot;current_class&quot;:&quot;bt_bb_padding_normal&quot;,&quot;def&quot;:&quot;normal&quot;}}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><\/div><\/div><\/div><div  class=\"bt_bb_column col-xxl-6 col-xl-6 bt_bb_vertical_align_middle bt_bb_align_left bt_bb_padding_20\" style=\"background-color:rgba(255, 255, 255, 1);; --column-width:6;\" data-width=\"6\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_border_color_none bt_bb_bottom_spacing_large\" data-bt-override-class=\"null\"><\/div><header class=\"bt_bb_headline bt_bb_color_scheme_8 bt_bb_dash_none bt_bb_superheadline bt_bb_subheadline bt_bb_size_normal bt_bb_align_inherit\" style=\"; --primary-color:#273033; --secondary-color:#ffffff;\" data-bt-override-class=\"{}\"><h2 class=\"bt_bb_headline_tag\"><span class=\"bt_bb_headline_superheadline\">IVIS TECHNOLOGY<\/span><span class=\"bt_bb_headline_content\"><span><em>In vivo<\/em> imaging system<\/span><\/span><\/h2><div class=\"bt_bb_headline_subheadline\">IVIS is a technology that allows for molecules monitoring, analyzing the distribution of drugs, and observing the kinetics of tumour growth on in vivo animals and in a non-invasive way.<\/div><\/header><div class=\"bt_bb_separator bt_bb_border_style_solid bt_bb_border_color_none bt_bb_top_spacing_normal bt_bb_bottom_spacing_normal\" data-bt-override-class=\"null\"><\/div><div  class=\"bt_bb_text\" ><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-3900 alignleft\" src=\"https:\/\/www.plaisant.eu\/wp-content\/uploads\/2022\/08\/ivis-unit-1.jpg\" alt=\"Plaisant - Animal Facility - Roma\" width=\"188\" height=\"188\" title=\"Plaisant - Animal Facility -\" srcset=\"https:\/\/www.plaisant.eu\/wp-content\/uploads\/2022\/08\/ivis-unit-1.jpg 499w, https:\/\/www.plaisant.eu\/wp-content\/uploads\/2022\/08\/ivis-unit-1-160x160.jpg 160w, https:\/\/www.plaisant.eu\/wp-content\/uploads\/2022\/08\/ivis-unit-1-320x320.jpg 320w\" sizes=\"(max-width: 188px) 100vw, 188px\" \/>Using this technology it is possible to <span>repeatedly <\/span>analyse the same live animal over time, instead of sacrificing many individuals to harvest and analyse tumours and organs, thus reducing the number of animals used in an experiment, and respecting the 3R principles.<\/p>\n<p>\u2014<\/p>\n<p><strong>Our IVIS SpectrumBL tool can be used for both bioluminescence and fluorescence in 3D.<\/strong><\/p>\n<p>\n<\/div><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_border_color_none bt_bb_bottom_spacing_large\" data-bt-override-class=\"null\"><\/div><\/div><\/div><\/div><div  class=\"bt_bb_column col-xxl-3 col-xl-3 bt_bb_vertical_align_top bt_bb_align_left bt_bb_padding_normal bt_bb_details_left bt_bb_details-color_white\" style=\"; --column-width:3;\" data-width=\"3\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section><section id=\"bt_bb_section69daac99471fb\" data-parallax=\"0\" data-parallax-offset=\"0\" class=\"bt_bb_section bt_bb_layout_wide bt_bb_vertical_align_top bt_bb_parallax\"   data-bt-override-class=\"null\"><div class=\"bt_bb_port\"><div class=\"bt_bb_cell\"><div class=\"bt_bb_cell_inner\"><div class=\"bt_bb_row bt_bb_row_push_right bt_bb_row_push_left bt_bb_content_wide bt_bb_row_width_boxed_1200 bt_bb_row_width_boxed  bt_bb_animation_fade_in move_left animate\"  data-bt-override-class=\"{}\"><div class=\"bt_bb_row_holder\" ><div  class=\"bt_bb_column col-xxl-3 col-xl-3 bt_bb_vertical_align_top bt_bb_align_left bt_bb_padding_normal bt_bb_details_right bt_bb_details-color_grey\" style=\"; --column-width:3;\" data-width=\"3\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><\/div><\/div><\/div><div  class=\"bt_bb_column col-xxl-6 col-xl-6 bt_bb_color_scheme_16 bt_bb_vertical_align_middle bt_bb_align_left bt_bb_padding_20 bt_bb_details-color_white\" style=\"; --column-primary-color:#191919; --column-secondary-color:#eaeef3;; --column-width:6;\" data-width=\"6\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_border_color_none bt_bb_bottom_spacing_large\" data-bt-override-class=\"null\"><\/div><header class=\"bt_bb_headline bt_bb_dash_none bt_bb_size_normal bt_bb_align_inherit\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_normal&quot;,&quot;def&quot;:&quot;normal&quot;,&quot;xl&quot;:&quot;normal&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;,&quot;xl&quot;:&quot;inherit&quot;}}\"><h3 class=\"bt_bb_headline_tag\"><span class=\"bt_bb_headline_content\"><span>FAQ<\/span><\/span><\/h3><\/header><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_normal\" data-bt-override-class=\"null\"><\/div><div class=\"bt_bb_accordion bt_bb_color_scheme_5 bt_bb_style_simple bt_bb_shape_square\" style=\"; --accordion-primary-color:#191919; --accordion-secondary-color:#018836;\" data-closed=closed><div class=\"bt_bb_accordion_item btWithIcon\"><div class=\"bt_bb_accordion_item_title_content\"><span  data-ico-icon7stroke=\"&#xe689;\" class=\"bt_bb_icon_holder\"><\/span><div class=\"bt_bb_accordion_item_title\">How does it work?<\/div><\/div><div class=\"bt_bb_accordion_item_content\"><div  class=\"bt_bb_text\" >\n<p style=\"font-weight: 400\">The IVIS \u00ae Spectrum in vivo imaging system combines 2D and 3D optical tomography in a single platform. The system uses state-of-the-art optical technology for research and development of preclinical images, ideal for non-invasive longitudinal monitoring of disease progression, cell traffic and patterns of gene expression in live animals.<\/p>\n<\/div>\n<\/div><\/div><div class=\"bt_bb_accordion_item btWithIcon\"><div class=\"bt_bb_accordion_item_title_content\"><span  data-ico-icon7stroke=\"&#xe689;\" class=\"bt_bb_icon_holder\"><\/span><div class=\"bt_bb_accordion_item_title\">What&#039;s the alternative?<\/div><\/div><div class=\"bt_bb_accordion_item_content\"><div  class=\"bt_bb_text\" >\n<p style=\"font-weight: 400\">Bioimaging allows for time kinetic studies on the same live animal. The alternative is to use more animals, to be sacrificed at different time points.<\/p>\n<\/div>\n<\/div><\/div><div class=\"bt_bb_accordion_item btWithIcon\"><div class=\"bt_bb_accordion_item_title_content\"><span  data-ico-icon7stroke=\"&#xe689;\" class=\"bt_bb_icon_holder\"><\/span><div class=\"bt_bb_accordion_item_title\">Is it safe for animals?<\/div><\/div><div class=\"bt_bb_accordion_item_content\"><div  class=\"bt_bb_text\" >\n<p style=\"font-weight: 400\">Mice are anaesthetised by gaseous anaesthesia (isoflurane) for up to 3-5 minutes and observed. Isoflurane is rapidly metabolised and does not cause any suffering to the animal.<\/p>\n<\/div>\n<\/div><\/div><div class=\"bt_bb_accordion_item btWithIcon\"><div class=\"bt_bb_accordion_item_title_content\"><span  data-ico-icon7stroke=\"&#xe689;\" class=\"bt_bb_icon_holder\"><\/span><div class=\"bt_bb_accordion_item_title\">What are the features and benefits?<\/div><\/div><div class=\"bt_bb_accordion_item_content\"><div  class=\"bt_bb_text\" >\n<ul>\n<li>In vivo imaging with high sensitivity of fluorescence and bioluminescence<\/li>\n<li>High performance (5 mice) with 23 cm field of view<\/li>\n<li>High resolution (up to 20 microns) with 3.9 cm field of view<\/li>\n<li>Twenty-eight high efficiency filters covering 430 \u2013 850 nm<\/li>\n<li>Supports spectral unmixing applications<\/li>\n<li>Ability to distinguish multiple bioluminescent and fluorescent reporters<\/li>\n<li>The optical switch in the fluorescent lighting path allows lighting in reflection mode or transmission mode<\/li>\n<li>Diffuse tomographic reconstruction 3D both for fluorescence and bioluminescence<\/li>\n<li>NIST Traceable Absolute Calibrations<\/li>\n<li>Entrance and exit doors for gaseous anaesthesia<\/li>\n<li>Class I lasers<\/li>\n<\/ul>\n<\/div>\n<\/div><\/div><\/div><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_border_color_none bt_bb_bottom_spacing_large\" data-bt-override-class=\"null\"><\/div><\/div><\/div><\/div><div data-bb-version=\"4.5.4\"  class=\"bt_bb_column col-xxl-3 col-xl-3 bt_bb_vertical_align_top bt_bb_align_left btLazyLoadBackground bt_bb_column_background_image bt_bb_padding_normal bt_bb_details_left-inner bt_bb_details-color_grey\" style=\"position: relative;background-image:url(&#039;https:\/\/www.plaisant.eu\/wp-content\/plugins\/bold-page-builder\/img\/blank.gif&#039;);; --column-width:3;\" data-background_image_src='https:\/\/www.plaisant.eu\/wp-content\/uploads\/2023\/06\/PLAISANT-ROMA.jpg' data-width=\"3\" data-bt-override-class=\"{&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_left&quot;,&quot;def&quot;:&quot;left&quot;},&quot;bt_bb_padding_&quot;:{&quot;current_class&quot;:&quot;bt_bb_padding_normal&quot;,&quot;def&quot;:&quot;normal&quot;}}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section><section id=\"bt_bb_section69daac99479a3\" data-parallax=\"0\" data-parallax-offset=\"0\" class=\"bt_bb_section bt_bb_color_scheme_1 bt_bb_layout_boxed_1200 bt_bb_vertical_align_top bt_bb_parallax bt_bb_background_image bt_bb_top_spacing_large bt_bb_bottom_spacing_large bt_bb_section_with_top_coverage_image bt_bb_section_with_bottom_coverage_image\" style=\"background-image:url(&#039;https:\/\/www.plaisant.eu\/wp-content\/uploads\/2020\/04\/background_2_single_service-3.jpg&#039;);\"  data-bt-override-class=\"{&quot;bt_bb_top_spacing_&quot;:{&quot;current_class&quot;:&quot;bt_bb_top_spacing_large&quot;,&quot;def&quot;:&quot;large&quot;,&quot;xl&quot;:&quot;large&quot;},&quot;bt_bb_bottom_spacing_&quot;:{&quot;current_class&quot;:&quot;bt_bb_bottom_spacing_large&quot;,&quot;def&quot;:&quot;large&quot;,&quot;xl&quot;:&quot;large&quot;}}\"><div class=\"bt_bb_port\"><div class=\"bt_bb_cell\"><div class=\"bt_bb_cell_inner\"><div class=\"bt_bb_row \"  data-bt-override-class=\"{}\"><div class=\"bt_bb_row_holder\" ><div  class=\"bt_bb_column col-xxl-5 col-xl-5 bt_bb_vertical_align_top bt_bb_align_left bt_bb_padding_normal\" style=\"; --column-width:5;\" data-width=\"5\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><\/div><\/div><\/div><div  class=\"bt_bb_column col-xxl-7 col-xl-7 bt_bb_vertical_align_middle bt_bb_align_left bt_bb_padding_normal bt_bb_animation_fade_in animate\" style=\"; --column-width:7;\" data-width=\"7\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_border_color_none bt_bb_bottom_spacing_medium\" data-bt-override-class=\"null\"><\/div><header class=\"bt_bb_headline bt_bb_font_weight_medium bt_bb_dash_none bt_bb_size_large bt_bb_align_inherit\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_large&quot;,&quot;def&quot;:&quot;large&quot;,&quot;xl&quot;:&quot;large&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;,&quot;xl&quot;:&quot;inherit&quot;}}\"><h1 class=\"bt_bb_headline_tag\"><span class=\"bt_bb_headline_content\"><span>Plaisant is always at the forefront of new frontiers in science.<\/span><\/span><\/h1><\/header><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_normal\" data-bt-override-class=\"null\"><\/div><div class=\"bt_bb_button bt_bb_color_scheme_6 bt_bb_icon_position_left bt_bb_style_filled bt_bb_size_normal bt_bb_width_inline bt_bb_shape_inherit bt_bb_target_self bt_bb_align_inherit\" style=\"; --primary-color:#ffffff; --secondary-color:#018836;\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_normal&quot;,&quot;def&quot;:&quot;normal&quot;,&quot;xl&quot;:&quot;normal&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;,&quot;xl&quot;:&quot;inherit&quot;}}\"><a href=\"\/en\/services\" target=\"_self\" class=\"bt_bb_link\" title=\"See all Services\"><span class=\"bt_bb_button_text\" >See all Services<\/span><span  data-ico-test=\"&#xf113;\" class=\"bt_bb_icon_holder\"><\/span><\/a><\/div><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_medium\" data-bt-override-class=\"null\"><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><div class=\"bt_bb_section_top_section_coverage_image\"><img decoding=\"async\" src=\"https:\/\/www.plaisant.eu\/wp-content\/uploads\/2022\/08\/top_cover_02-2.png\" alt=\"bt_bb_section_top_section_coverage_image\" title=\"Plaisant - Animal Facility -\"><\/div><div class=\"bt_bb_section_bottom_section_coverage_image\"><img decoding=\"async\" src=\"https:\/\/www.plaisant.eu\/wp-content\/uploads\/2020\/04\/bottom_cover_3-2.png\" alt=\"bt_bb_section_bottom_section_coverage_image\" title=\"Plaisant - Animal Facility -\"><\/div><\/section><section id=\"bt_bb_section69daac9947f65\" data-parallax=\"0.7\" data-parallax-offset=\"0\" data-background_image_src=\"'https:\/\/www.plaisant.eu\/wp-content\/uploads\/2020\/05\/background_02-1.png'\" class=\"bt_bb_section bt_bb_color_scheme_10 bt_bb_layout_wide bt_bb_vertical_align_top bt_bb_parallax btLazyLoadBackground bt_bb_background_image bt_bb_background_overlay_accent_bottom_gradient bt_bb_top_spacing_large\" style=\"background-image:url(&#039;https:\/\/www.plaisant.eu\/wp-content\/plugins\/bold-page-builder\/img\/blank.gif&#039;);;background-color:#091357;\"  data-bt-override-class=\"null\"><div class=\"bt_bb_port\"><div class=\"bt_bb_cell\"><div class=\"bt_bb_cell_inner\"><div class=\"bt_bb_row bt_bb_row_width_boxed_1200 bt_bb_row_width_boxed \"  data-bt-override-class=\"{}\"><div class=\"bt_bb_row_holder\" ><div  class=\"bt_bb_column col-xxl-12 col-xl-12 bt_bb_vertical_align_top bt_bb_align_left bt_bb_padding_normal bt_bb_animation_fade_in animate\" style=\"; --column-width:12;\" data-width=\"12\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><div data-bb-version=\"4.5.4\" class=\"bt_bb_separator btWithText bt_bb_border_style_solid bt_bb_border_color_light bt_bb_top_spacing_ bt_bb_bottom_spacing_medium\" data-bt-override-class=\"{&quot;bt_bb_top_spacing_&quot;:{&quot;current_class&quot;:&quot;bt_bb_top_spacing_&quot;,&quot;def&quot;:&quot;&quot;},&quot;bt_bb_bottom_spacing_&quot;:{&quot;current_class&quot;:&quot;bt_bb_bottom_spacing_medium&quot;,&quot;def&quot;:&quot;medium&quot;}}\"><span class=\"bt_bb_separator_text\">Hours of Plaisant<\/span><\/div><\/div><\/div><\/div><\/div><\/div><div class=\"bt_bb_row bt_bb_row_width_boxed_1200 bt_bb_row_width_boxed \"  data-bt-override-class=\"{}\"><div class=\"bt_bb_row_holder\" ><div  class=\"bt_bb_column col-xxl-2_4 col-xl-2_4 col-xs-12 col-sm-12 col-md-12 col-lg-2_4 bt_bb_vertical_align_top bt_bb_align_center bt_bb_padding_normal bt_bb_animation_fade_in animate\" style=\"; --column-width:2.4;\" data-width=\"2.4\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><header data-bb-version=\"4.5.4\" class=\"bt_bb_headline bt_bb_subheadline_text_transform_default bt_bb_color_scheme_1 bt_bb_dash_none bt_bb_subheadline bt_bb_size_extrasmall bt_bb_align_inherit\" style=\"; --primary-color:#ffffff; --secondary-color:#191919;\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_extrasmall&quot;,&quot;def&quot;:&quot;extrasmall&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;}}\"><h4 class=\"bt_bb_headline_tag\"><span class=\"bt_bb_headline_content\"><span>Mon<\/span><\/span><\/h4><div class=\"bt_bb_headline_subheadline\">8:00 - 17:00<\/div><\/header><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_medium\" data-bt-override-class=\"null\"><\/div><\/div><\/div><\/div><div  class=\"bt_bb_column col-xxl-2_4 col-xl-2_4 col-xs-12 col-sm-12 col-md-12 col-lg-2_4 bt_bb_vertical_align_top bt_bb_align_center bt_bb_padding_normal bt_bb_animation_fade_in animate\" style=\"; --column-width:2.4;\" data-width=\"2.4\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><header data-bb-version=\"4.5.4\" class=\"bt_bb_headline bt_bb_subheadline_text_transform_default bt_bb_color_scheme_1 bt_bb_dash_none bt_bb_subheadline bt_bb_size_extrasmall bt_bb_align_inherit\" style=\"; --primary-color:#ffffff; --secondary-color:#191919;\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_extrasmall&quot;,&quot;def&quot;:&quot;extrasmall&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;}}\"><h4 class=\"bt_bb_headline_tag\"><span class=\"bt_bb_headline_content\"><span>Tues<\/span><\/span><\/h4><div class=\"bt_bb_headline_subheadline\">8:00 - 17:00<\/div><\/header><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_medium\" data-bt-override-class=\"null\"><\/div><\/div><\/div><\/div><div  class=\"bt_bb_column col-xxl-2_4 col-xl-2_4 col-xs-12 col-sm-12 col-md-12 col-lg-2_4 bt_bb_vertical_align_top bt_bb_align_center bt_bb_padding_normal bt_bb_animation_fade_in animate\" style=\"; --column-width:2.4;\" data-width=\"2.4\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><header data-bb-version=\"4.5.4\" class=\"bt_bb_headline bt_bb_subheadline_text_transform_default bt_bb_color_scheme_1 bt_bb_dash_none bt_bb_subheadline bt_bb_size_extrasmall bt_bb_align_inherit\" style=\"; --primary-color:#ffffff; --secondary-color:#191919;\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_extrasmall&quot;,&quot;def&quot;:&quot;extrasmall&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;}}\"><h4 class=\"bt_bb_headline_tag\"><span class=\"bt_bb_headline_content\"><span>Wed<\/span><\/span><\/h4><div class=\"bt_bb_headline_subheadline\">8:00 - 17:00<\/div><\/header><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_medium\" data-bt-override-class=\"null\"><\/div><\/div><\/div><\/div><div  class=\"bt_bb_column col-xxl-2_4 col-xl-2_4 col-xs-12 col-sm-12 col-md-12 col-lg-2_4 bt_bb_vertical_align_top bt_bb_align_center bt_bb_padding_normal bt_bb_animation_fade_in animate\" style=\"; --column-width:2.4;\" data-width=\"2.4\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><header data-bb-version=\"4.5.4\" class=\"bt_bb_headline bt_bb_subheadline_text_transform_default bt_bb_color_scheme_1 bt_bb_dash_none bt_bb_subheadline bt_bb_size_extrasmall bt_bb_align_inherit\" style=\"; --primary-color:#ffffff; --secondary-color:#191919;\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_extrasmall&quot;,&quot;def&quot;:&quot;extrasmall&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;}}\"><h4 class=\"bt_bb_headline_tag\"><span class=\"bt_bb_headline_content\"><span>Thurs<\/span><\/span><\/h4><div class=\"bt_bb_headline_subheadline\">8:00 - 17:00<\/div><\/header><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_medium\" data-bt-override-class=\"null\"><\/div><\/div><\/div><\/div><div  class=\"bt_bb_column col-xxl-2_4 col-xl-2_4 col-xs-12 col-sm-12 col-md-12 col-lg-2_4 bt_bb_vertical_align_top bt_bb_align_center bt_bb_padding_normal bt_bb_animation_fade_in animate\" style=\"; --column-width:2.4;\" data-width=\"2.4\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><header data-bb-version=\"4.5.4\" class=\"bt_bb_headline bt_bb_subheadline_text_transform_default bt_bb_color_scheme_1 bt_bb_dash_none bt_bb_subheadline bt_bb_size_extrasmall bt_bb_align_inherit\" style=\"; --primary-color:#ffffff; --secondary-color:#191919;\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_extrasmall&quot;,&quot;def&quot;:&quot;extrasmall&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;}}\"><h4 class=\"bt_bb_headline_tag\"><span class=\"bt_bb_headline_content\"><span>Fri<\/span><\/span><\/h4><div class=\"bt_bb_headline_subheadline\">8:00 - 17:00<\/div><\/header><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_medium\" data-bt-override-class=\"null\"><\/div><\/div><\/div><\/div><\/div><\/div><div class=\"bt_bb_row \"  data-bt-override-class=\"{}\"><div class=\"bt_bb_row_holder\" ><div  class=\"bt_bb_column col-xxl-12 col-xl-12 bt_bb_vertical_align_top bt_bb_align_left bt_bb_padding_normal\" style=\"; --column-width:12;\" data-width=\"12\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_medium\" data-bt-override-class=\"null\"><\/div><\/div><\/div><\/div><\/div><\/div><div class=\"bt_bb_row bt_bb_row_push_right bt_bb_row_push_left bt_bb_content_wide bt_bb_row_width_boxed_1200 bt_bb_row_width_boxed  bt_bb_animation_fade_in move_right animate\"  data-bt-override-class=\"{}\"><div class=\"bt_bb_row_holder\" ><div data-bb-version=\"4.5.4\"  class=\"bt_bb_column col-xxl-3 col-xl-3 bt_bb_vertical_align_top bt_bb_align_left btLazyLoadBackground bt_bb_column_background_image bt_bb_padding_normal bt_bb_details_right-inner bt_bb_details-color_white\" style=\"background-image:url(&#039;https:\/\/www.plaisant.eu\/wp-content\/plugins\/bold-page-builder\/img\/blank.gif&#039;);; --column-width:3;\" data-background_image_src='https:\/\/www.plaisant.eu\/wp-content\/uploads\/2023\/06\/PLAISANT-CASTEL-ROMANO-11.jpg' data-width=\"3\" data-bt-override-class=\"{&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_left&quot;,&quot;def&quot;:&quot;left&quot;},&quot;bt_bb_padding_&quot;:{&quot;current_class&quot;:&quot;bt_bb_padding_normal&quot;,&quot;def&quot;:&quot;normal&quot;}}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><\/div><\/div><\/div><div  class=\"bt_bb_column col-xxl-6 col-xl-6 bt_bb_color_scheme_2 bt_bb_vertical_align_middle bt_bb_align_left bt_bb_padding_20\" style=\"; --column-primary-color:#191919; --column-secondary-color:#ffffff;background-color:rgba(255, 255, 255, 1);; --column-width:6;\" data-width=\"6\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_border_color_none bt_bb_bottom_spacing_large\" data-bt-override-class=\"null\"><\/div><header class=\"bt_bb_headline bt_bb_dash_none bt_bb_superheadline bt_bb_size_normal bt_bb_align_inherit\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_normal&quot;,&quot;def&quot;:&quot;normal&quot;,&quot;xl&quot;:&quot;normal&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;,&quot;xl&quot;:&quot;inherit&quot;}}\"><h3 class=\"bt_bb_headline_tag\"><span class=\"bt_bb_headline_superheadline\">CONTACT US<\/span><span class=\"bt_bb_headline_content\"><span>Ask for information about the service<\/span><\/span><\/h3><\/header><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_normal\" data-bt-override-class=\"null\"><\/div><div data-bb-version=\"4.6.1\" class=\"bt_bb_contact_form_7\">\n<div class=\"wpcf7 no-js\" id=\"wpcf7-f4529-o1\" lang=\"it-IT\" dir=\"ltr\">\n<div class=\"screen-reader-response\"><p role=\"status\" aria-live=\"polite\" aria-atomic=\"true\"><\/p> <ul><\/ul><\/div>\n<form action=\"\/en\/wp-json\/wp\/v2\/pages\/4463#wpcf7-f4529-o1\" method=\"post\" class=\"wpcf7-form init\" aria-label=\"Modulo di contatto\" novalidate=\"novalidate\" data-status=\"init\">\n<div style=\"display: none;\">\n<input type=\"hidden\" name=\"_wpcf7\" value=\"4529\" \/>\n<input type=\"hidden\" name=\"_wpcf7_version\" value=\"5.9\" \/>\n<input type=\"hidden\" name=\"_wpcf7_locale\" value=\"it_IT\" \/>\n<input type=\"hidden\" name=\"_wpcf7_unit_tag\" value=\"wpcf7-f4529-o1\" \/>\n<input type=\"hidden\" name=\"_wpcf7_container_post\" value=\"0\" \/>\n<input type=\"hidden\" name=\"_wpcf7_posted_data_hash\" value=\"\" \/>\n<\/div>\n<div class=\"btRequest\">\n\t<div class=\"btRequestFlex\">\n\t\t<div class=\"btRequestFlexLeft\">\n\t\t\t<div class=\"btRequestLabel\">\n\t\t\t\t<p>Name and Surname (required)*\n\t\t\t\t<\/p>\n\t\t\t<\/div>\n\t\t\t<div class=\"btRequestInput\">\n\t\t\t\t<p><span class=\"wpcf7-form-control-wrap\" data-name=\"your-name\"><input size=\"40\" class=\"wpcf7-form-control wpcf7-text wpcf7-validates-as-required\" aria-required=\"true\" aria-invalid=\"false\" value=\"\" type=\"text\" name=\"your-name\" \/><\/span>\n\t\t\t\t<\/p>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t<div class=\"btRequestFlexLeft\">\n\t\t\t<div class=\"btRequestLabel\">\n\t\t\t\t<p>Email (required)*\n\t\t\t\t<\/p>\n\t\t\t<\/div>\n\t\t\t<div class=\"btRequestInput\">\n\t\t\t\t<p><span class=\"wpcf7-form-control-wrap\" data-name=\"your-email\"><input size=\"40\" class=\"wpcf7-form-control wpcf7-email wpcf7-validates-as-required wpcf7-text wpcf7-validates-as-email\" aria-required=\"true\" aria-invalid=\"false\" value=\"\" type=\"email\" name=\"your-email\" \/><\/span>\n\t\t\t\t<\/p>\n\t\t\t<\/div>\n\t\t<\/div>\n\t<\/div>\n\t<div class=\"btRequestLabel\">\n\t\t<p>Message\n\t\t<\/p>\n\t<\/div>\n\t<div class=\"btRequestInput\">\n\t\t<p><span class=\"wpcf7-form-control-wrap\" data-name=\"your-message\"><textarea cols=\"40\" rows=\"10\" class=\"wpcf7-form-control wpcf7-textarea\" aria-invalid=\"false\" name=\"your-message\"><\/textarea><\/span>\n\t\t<\/p>\n\t<\/div>\n\t<p><span class=\"wpcf7-form-control-wrap kc_captcha\" data-name=\"kc_captcha\"><span class=\"wpcf7-form-control wpcf7-radio\"><span class=\"captcha-image\" ><span class=\"cf7ic_instructions\">Si prega di selezionare sotto l'icona della parola<span> Automobile<\/span>.<\/span><label><input aria-label=\"1\" type=\"radio\" name=\"kc_captcha\" value=\"bot\" \/><svg aria-hidden=\"true\" role=\"img\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 576 512\"><path fill=\"currentColor\" d=\"M259 18l-65 132-146 22c-26 3-37 36-18 54l106 103-25 146c-5 26 23 46 46 33l131-68 131 68c23 13 51-7 46-33l-25-146 106-103c19-18 8-51-18-54l-146-22-65-132a32 32 0 00-58 0z\"\/><\/svg><\/label><label><input aria-label=\"2\" type=\"radio\" name=\"kc_captcha\" value=\"kc_human\" \/><svg aria-hidden=\"true\" role=\"img\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\"><path fill=\"currentColor\" d=\"M500 168h-55l-8-21a127 127 0 00-120-83H195a127 127 0 00-120 83l-8 21H12c-8 0-14 8-11 16l8 24a12 12 0 0011 8h29a64 64 0 00-33 56v48c0 16 6 31 16 42v62c0 13 11 24 24 24h48c13 0 24-11 24-24v-40h256v40c0 13 11 24 24 24h48c13 0 24-11 24-24v-62c10-11 16-26 16-42v-48c0-24-13-45-33-56h29a12 12 0 0011-8l8-24c3-8-3-16-11-16zm-365 2c9-25 33-42 60-42h122c27 0 51 17 60 42l15 38H120l15-38zM88 328a32 32 0 010-64c18 0 48 30 48 48s-30 16-48 16zm336 0c-18 0-48 2-48-16s30-48 48-48 32 14 32 32-14 32-32 32z\"\/><\/svg><\/label><label><input aria-label=\"3\" type=\"radio\" name=\"kc_captcha\" value=\"bot\" \/><svg aria-hidden=\"true\" role=\"img\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\"><path fill=\"currentColor\" d=\"M350 99c-54 0-98-35-166-35-25 0-47 4-68 12a56 56 0 004-24C118 24 95 1 66 0a56 56 0 00-34 102v386c0 13 11 24 24 24h16c13 0 24-11 24-24v-94c28-12 64-23 114-23 54 0 98 35 166 35 48 0 86-16 122-41 9-6 14-15 14-26V96c0-23-24-39-45-29-35 16-77 32-117 32z\"\/><\/svg><\/label>\r\n    <\/span>\r\n    <span style=\"display:none\">\r\n        <input type=\"text\" name=\"kc_honeypot\">\r\n    <\/span><\/span><\/span>\n\t<\/p>\n\t<div class=\"btRequestButton\">\n\t\t<p><input class=\"wpcf7-form-control wpcf7-submit has-spinner\" type=\"submit\" value=\"Send\" \/>\n\t\t<\/p>\n\t<\/div>\n<\/div><div class=\"wpcf7-response-output\" aria-hidden=\"true\"><\/div>\n<\/form>\n<\/div>\n<\/div><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_border_color_none bt_bb_bottom_spacing_large\" data-bt-override-class=\"null\"><\/div><\/div><\/div><\/div><div  class=\"bt_bb_column col-xxl-3 col-xl-3 bt_bb_vertical_align_top bt_bb_align_left bt_bb_padding_normal bt_bb_details_left bt_bb_details-color_white\" style=\"; --column-width:3;\" data-width=\"3\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":4338,"parent":4462,"menu_order":10,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"_links":{"self":[{"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/pages\/4463"}],"collection":[{"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/comments?post=4463"}],"version-history":[{"count":9,"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/pages\/4463\/revisions"}],"predecessor-version":[{"id":4885,"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/pages\/4463\/revisions\/4885"}],"up":[{"embeddable":true,"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/pages\/4462"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/media\/4338"}],"wp:attachment":[{"href":"https:\/\/www.plaisant.eu\/en\/wp-json\/wp\/v2\/media?parent=4463"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}